Population dynamics of teak defoliator, Hyblaea puera Cram. (Lep., Hyblaeidae) in teak plantations of Bangladesh, Journal of Applied Entomology, Volume Abstract. Hyblaea puera Cramer (Lepidoptera: Hyblaeidae) was reported in commercial teak (Tectona grandis L. f.) plantations in the states of Campeche and. Hyblaea puera Cramer (Lepidoptera: Hyblaeidae) was reported in commercial teak (Tectona grandis L. f.) plantations in the states of.

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An absolutely specific virus with refractile polyhedral inclusion bodies, staining blue in Phera and thick blue in Buffalo Blacknamed as Hyblaea puera nucleopolyhedrovirus HpNPV is found to be very effective in the biological control of this pest.

Slaves of the environment: The bands scored for each nuclear RAGEP used in the present study were of a size range bp to bp. This pest is of major concern as it is involved in complete defoliation of trees during the early part pueda the growing season. The average pupal period lasts six to eight days under optimal conditions. Duringan investigation of microbial hgblaea of H. Later the viral DNA takes over control of the cellular machinery to reproduce itself.

Assessing genetic structure with multiple classes of molecular markers: This suggests that all the epidemic insects, which are spatially distinct, but temporally co-occurring, share the same gene pool.

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The maximum number of monomorphic bands puwra observed using marker CB-N Teak Tectona grandis L. Using the mitochondrial RAGEP markers, the average numbers of bands scored for each primer ranged from 6— The present results appear to validate the hypothesis, that control of H. Normalization helped us to control the brightness and streakiness of bands without altering the lighter bands and also control the inter-gel mobility shifts. By using this site, you agree to the Terms of Use and Privacy Policy.

Moth Photographers Group – Hyblaea puera –

Blackwell publishing, Oxford, U. Daly JC, Gregg P. Furthermore, the impact of the caterpillars can result in forked tops if the damage affects the leaders. Real-time normalization of gel electrophoresis patterns and band position for all the gels was based on the reference system for the species-specific bands. All authors read and approved the final manuscript.


The NPV which enters the insect gut, lyses in the alkaline environment of the midgut, releasing virions. The ECVs which are the progenies released into the haemocoel from the midgut cells are more infectious than the PIBs and mediate disease spread within the insect body. The NPVs come under the family of baculoviridae and its virions are enveloped rod shaped nucleocapsids containing circular, supercoiled, double stranded DNA.

Even in the fully mature larvae, HpNPV can kill in 60—72 hours, making it one of the fastest acting insect viruses. DNA isolation DNA extraction was performed with a minor modification of isolation and purification protocol as described earlier [ 37 ] being extracted from whole larvae and quantified spectrophotometrically using a spectrophotometer at nm Shimadzu.

Distinct mitochondrial and nuclear DNA sequence divergence patterns for phylogenetic inference has been established among nymphalid butterflies [ 29 ]. The eggs are laid singly on the leaves of the foodplant.

Use of genetic diversity in movement studies of flying insects.

Genetic characterization pueera populations of a de novo arisen sugar beet pest, Aubeonymus mariaefranciscae Coleoptera, Curculionidaeby RAPD analysis. Using the duration of each instar egg — one day; 1 st and 2 nd instars — two days each, 3 rd to 5 th instars — three days each; pre-pupa — one day and pupa — four daysthe temporal data on outbreaks were examined to see whether each subsequent epidemic could be explained on the basis of hyblasa previous outbreak.

Predatory insects including wasps, spiders, birds and bonnet macaqueswhich are known to comprise the predator complex of H.

Apart from teak there is a large number of alternative host plants for these polyphagous caterpillars. Thailand and is of major economic significance. Retrieved 4 July Earlier reports involving molecular DNA markers mention the use of these markers in the detection of sibling species of black flies, Simulium spp. Such information should prove valuable in planning and implementing measures to control these pests.

Gene flow, phylogeography and their uses.

If only lower stages were available, i. The pupae are also hidden in leaf folds or in between leaves that are tied together. Geographic structure of insect populations: The maximum numbers of bands detected was found using primer SR-J, the minimum numbers ppuera marker N4-N From this dendrogram, it may be deduced that all the seven epidemic population samples tested in the study shared the same gene pool with sets of epicenter populations.


There are five larval instars. Hyblaea puera Cramer A DNA fingerprinting procedure for ultra high-throughput genetic analysis of insects. The amplification products were separated using 1.

Arbitrarily-primed DNA markers, and involving the polymerase chain reaction PCR hyb,aea, have proved very useful for genetic fingerprinting and for facilitating positional cloning of genes. From then on until the next year, the population remains at the endemic level. The Teak defoliator is of major concern since it is involved in complete defoliation of trees during the early part of the growing season.

The larval samples that were geographically close and had a difference of one complete life cycle stage between the population groups were subjected to molecular studies to evaluate their relatedness. Teak defoliator puefa are a regular annual feature in teak plantations in Kerala, India.

The dendrogram produced from nuclear RAGEP clearly indicates that the endemic insects are not involved in causing the epidemic; however, they are apparently involved in the localized spread by building up small epicenter populations. Thus the molecular markers detected using RAGEP-PCR can enhance the understanding of insect population dynamics and aid in tracing the spread and cause of epidemics. We were therefore interested in investigating the temporal and spatial relationship among various population groups in Nilambur, Kerala India and address the cause of outbreak at the landscape level.

University of York, UK; Larvae hatch in about two days. Since the damage is very severe and effective control measures are unavailable, the large scale planting of teak has been abandoned in PNG.